SEM Instructions

Your work with the Scanning Electron Microscope at the Pitt Center for Biologic Imaging will not be evaluated, but it will be checked (pass/fail).

Produce the following image sets:

  • An image of your sample at millimeter scale (~a “familiar view”)
  • An image of your sample at micrometer or nanometer scale (~an “unfamiliar view”)
  • We will be making an anaglyphic (red/cyan) 3D stereo view of your SEM project. In order to support this, you should record one or more stereo pairs: a pair of images of your subject taken from a short distance apart. You’ll need to tinker with getting the right stereo disparity (too little distance between the images and there won’t be enough depth, too much and it will be difficult for the viewer to resolve/fuse). It may be best to record a sequence of ~5 images, each slightly moved/rotated from the last, and then select the best stereo pair.

Please deliver your work as follows:

  • Create a blog post.
  • Categorize your blog post with the WordPress category, SEM.
  • Retrieve your SEM images (in TIFF format) from the CBI lab’s online downloads website; a link will be provided to you.
  • Convert the SEM images to JPG format with minimal compression, using Photoshop or something similar. Upload and publish them in the blog post, making sure that you link to the highest-resolution versions.
  • Please describe what we’re seeing in the images: what did you scan?
  • Also: publish your stereo pair(s) as individual images in the blog. A workflow will be provided at a later date for combining these into an anaglyphic display.
  • Write 100 words (approximately a paragraph) describing your experience, and some things you learned.

To Do for Thursday 1/23

  • To read: Read this Typologies article.
  • To do: Review five of the “Project to Share” posts by your peers. Select one; in a blog post:
    1. Write a brief response that adds something.
    2. (List the other posts you reviewed, too.)

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